Standard Operating Procedure

Title:DNA Extraction from Soybean Leaves

Dept:Agronomy

Lab:Agronomy G313, B411, B406

Supervisor:Randy Shoemaker

1. Know the location of the Material Safety Data Sheets (MSDS) for all hazardous chemicals used in this

procedure (chloroform, 2-mercaptoethanol, CTAB, isopropanol and isoamyl alcohol). Read and become

familiar with the safe use of these chemicals. If you have a question or concern regarding health or safety with

respect to a specific chemical, consult the laboratory supervisor, or the principal investigator before

proceeding.

2. Always use protective clothing when performing this procedure. This means wear a laboratory coat, goggles

and Nitrile gloves (not latex) when handling chloroform.

3. Always use chloroform and 2-mercaptoethanol in a fume hood. When working in the fume hood, always be

certain the fan is on and the sash is lowered to the correct level as indicated by the arrows on the frame and

sash.

4. Whenever you have a question or concern regarding health or safety with respect to a specific procedure,

consult the laboratory supervisor or the principal investigator before proceeding.