Standard Operating Procedure

Title:Protein variant gels using Acrylamide: Sample prep, setting gel, electrophoresis, and visualization

Dept: Agronomy

Lab: Agronomy G313, B411, B413

Supervisor: Randy Shoemaker

1. Know the location of the Material Safety Data Sheet (MSDS) for all hazardous chemicals used in this
procedure (acrylamide, bis-acrylamide, SDS, ammonium persulfate, TEMED, _-mercaptoethanol, urea, Tris-
base, HCl, glycine, methanol, glacial acetate, coomassie blue, bromophenol blue, glycerol). Read and
become familiar with the safe use of these chemicals. If you have a question or concern regarding health or
safety with respect to a specific chemical, consult the laboratory supervisor, or the principal investigator before
proceeding.

2. Always wear gloves and lab coat when handling the acrylamide in any form. In addition, wear goggles and
particle mask when handling crystal acrylamide.

3. Be careful with the razor blade you are using to obtain the seed storage protein sample. The razor blade is
sharp and your thumb is likely to be nicked if you are not careful.

4. Be careful around the gel tanks while the power is on. These gels run at considerable voltage.