Standard Operating Procedure

Title:Fluorometeric quanfification of DNA

Dept: Agronomy

Lab: Agronomy G313, B411, B413

Supervisor: Randy Shoemaker

1. Know the location of the Material Safety Data Sheets (MSDS) for all hazardous chemicals used in this

procedure (TNE (Tris, NaCl, and EDTA) and Hoechst dye (benzoxanthene yellow-if looking for this on the web,

you may need to go to the Sigma MSDS page)). Read and become familiar with the safe use of these

chemicals. If you have a question or concern regarding health or safety with respect to a specific chemical,

consult the laboratory supervisor, or the principal investigator before proceeding.

2. Protective clothing required includes a laboratory coat and latex gloves, and safety glasses.

3. Whenever you have a question or concern regarding health or safety with respect to a specific procedure,

consult the laboratory supervisor or the principal investigator before proceeding.