A bacterial artificial chromosome library for soybean and identification of clones near a major cyst nematode resistance gene.

Reference Report for IND21643571

Title:	A bacterial artificial chromosome library for soybean and identification of clones near a major cyst nematode resistance gene.
Authors:	Danesh, D., Penuela, S., Mudge, J., Denny, R.L., Nordstrom, H., Martinez, J.P., Young, N.D.
Source:	Theor. Appl. Genet. 1998, 96(2):196-202
Abstract:	A bacterial artificial chromosome (BAC) library was constructed for soyabean (Glycine max) consisting of approximately 30000 clones with an average insert size of 120 kilobase pairs. The library was successfully screened with restriction fragment length polymorphism (RFLP) and microsatellite markers tightly linked to a major resistance gene for the cyst nematode, Heterodera glycines. Since many soyabean RFLPs hybridize to duplicate loci, BACs homologous to duplicate RFLP loci were distinguished by digestion with the restriction enzyme originally used to map the RFLP, followed by a comparison of the hybridizing fragments. Linkage mapping of BAC clones identified with markers linked to the cyst nematode resistance gene demonstrated that these clones were located at the expected chromosomal positions and that there were no indications of chimaeras within the genomic inserts

Funded by the USDA-ARS. Developed by the USDA-ARS SoyBase and Legume Clade Database group at the Iowa State University, Ames, IA

U.S. Public Domain