Reference Report for IND21984884
| Title: | Molecular markers residing close to the Rhg4 locus conferring resistance to soybean cyst nematode race 3 on linkage group A of soybean. |
| Authors: | Matthews, B.F., MacDonald, M.H., Gebhardt, J.S., Devine, T.E. |
| Source: | Theor. Appl. Genet. 1998, 97(7):1047-1052 |
| Abstract: | The restriction fragment length polymorphism (RFLP) clone pBLT65 is a 450-nt soybean cDNA encoding a portion of the bifunctional enzyme aspartokinase-homoserine dehydrogenase (AK-HSDH). pBLT65 maps within 3.5 cM of the i locus, conferring a pigmented seed coat, on linkage group A, hence, it is closely linked to the Rhg4 locus conferring resistance to race 3 of the soybean cyst nematode. From this useful RFLP we developed a PCR reaction yielding polymorphic bands for use in marker-assisted breeding programs to select progeny containing the Rhg4 allele. The polymorphic bands were sequenced to determine the cause of the polymorphisms. Using primers 548 and 563, PCR amplification of DNA from the soybean cultivar Peking (Rhg4) yielded three DNA fragments, 1a (1160 bp), 1b (1146 bp) and 3 (996 bp). Amplification of DNA from the cultivar Kent (rhg4) yielded DNA fragments 2 (1020 bp) 3 (996 bp) and 4 (960 bp). Fragments 1a. 1b, 2 and 4 were also polymorphic between the soybean lines PI 290136 and BARC-2(Rj4). A segregating population of 80 F2 and F3 plants derived from the cross PI 290136xBARC-2 (Rj4) was used to confirm the map position of the PCR polymorphisms near the i locus, and hence the Rhg4 locus on linkage group A. The nucleotide sequences of fragments 1b, 3 and 4 were determined. Large and small deletions in the intronic region were responsible for the size differences of the different fragments, where as the exon was well conserved. |
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