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Reference Report for IND80037692
Title:Immunochemical and microscopical techniques for detecting blackeye cowpea mosaic and soybean mosaic viruses in hypocotyls of germinated seeds.
Authors:Lima, J.A.A., Purcifull, D.E.
Source:Phytopath. 1980, 70(2):142-147
Abstract:Double immunodiffusion tests were used to detect BlCMV in hypocotyls of infected, 4- to 5-day-old cowpea seedlings and SMV in soybean hypocotyls. Antigens consisted either of hypocotyl extracts prepared in sodium dodecyl sulfate (SDS) or of hypocotyl disks embedded in agar media containing SDS. The percentages of infected seed in 5 lots of cowpea seed and 1 lot of soybean seed, as determined by the hypocotyl disk immunodiffusion test, were similar to the percentages of infection determined for these same lots by detecting symptoms in seedlings. Serologically specific electron microscopy was also used to detect both viruses in infected hypocotyls. Cytoplasmic (cylindrical) inclusions were detected in BlCMV infected cowpea hypocotyls and SMV infected soybean hypocotyls by light microscopy, electron microscopy and serology.






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