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Integrating Genetics and Genomics to Advance Soybean Research

Reference Report for KGK20170518.1
Title:Mapping quantitative trait loci for root development under hypoxia conditions in soybean (Glycine max L. Merr.)
Authors:Nguyen, L., Takahashi, R., Githiri, S. , Rodriguez, T., Tsutsumi, N., Kajihara, S., Sayama, T., Ishimoto, M., Harada, K, Suematsu, K., Abiko, T., Mochizuki, T.
Source:Theor Appl Genet. 2017, 130(4):743-755
Abstract:Waterlogging is a major environmental stress limiting soybean yield in wet parts of the world. Root development is an important indicator of hypoxia toler- ance in soybean. However, little is known about the genetic control of root development under hypoxia. This study was conducted to identify quantitative trait loci (QTLs) responsible for root development under hypoxia. Recombi- nant inbred lines (RILs) developed from a cross between a hypoxia-sensitive cultivar, Tachinagaha, and a tolerant landrace, Iyodaizu, were used. Seedlings were subjected to hypoxia, and root development was evaluated with the value change in root traits between after and before treat- ments. We found 230 polymorphic markers spanning 2519.2 cM distributed on all 20 chromosomes (Chrs.). Using these, we found 11 QTLs for root length (RL), root length development (RLD), root surface area (RSA), root surface area development (RSAD), root diameter (RD), and change in average root diameter (CARD) on Chrs. 11, 12, 13 and 14, and 7 QTLs for hypoxia tolerance of these root traits. These included QTLs for RLD and RSAD between markers Satt052 and Satt302 on Chr. 12, which are impor- tant markers of hypoxia tolerance in soybean; those QTLs were stable between 2 years. To validate the QTLs, we developed a near-isogenic line with the QTL region derived from Iyodaizu. The line performed well under both hypoxia and waterlogging, suggesting that the region contains one or more genes with large e ects on root development. These ndings may be useful for ne mapping and posi- tional cloning of gene responsible for root development under hypoxia.






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