Methylation Changes in Soybean Early Maturation Seed Parts

Reference Report for RTN20151123.3

Title:	Methylation Changes in Soybean Early Maturation Seed Parts
Authors:	Goldberg, R.B., Chen, M., Lin, J., Le, B.H.
Source:	Unpublished Dataset
Abstract:	Seeds are comprised of three majors parts of distinct parental origin: the seed coat, embryo, and endosperm. The maternally-derived seed coat is important for nurturing and protecting the seeds during development. By contrast, the embryo and the endosperm are derived from a double fertilization event, where one sperm fertilizes the egg to form the diploid zygote and the other sperm fertilizes the central cell to form the triploid endosperm. Each seed parts undergo distinct developmental programs during seed development. What methylation changes occurring in the different seed parts, if any, remains unknown. To uncover the possible role of DNA methylation in different parts of the seed, we characterized the methylome of three major parts of an early maturation stage seed: seed coat, embryonic cotyledons, and embryonic axis using Illumina sequencing. To correlate DNA methylation with gene expression and small RNAs, we characterized the transcriptome and small RNAs of three major parts of an early maturation stage seed.

Funded by the USDA-ARS. Developed by the USDA-ARS SoyBase and Legume Clade Database group at the Iowa State University, Ames, IA

U.S. Public Domain