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Integrating Genetics and Genomics to Advance Soybean Research

Reference Report for RTN20151123.7
Title:Methylation Changes During Soybean Seed Development
Authors:Goldberg, R.B., Chen, M., Lin, J., Le, B.H.
Source:Unpublished Dataset
Abstract:To uncover the possible role of DNA methylation throughout all of seed development - from fertilization through dormancy and post-germination in soybean, we characterized the methylome of whole seeds representing the differentiation (GLOB and COT stages), maturation (early- [EM], mid- [MM] and late- [LM] maturation stages), pre-dormancy (PD1 and PD2 stages), dormancy (DRY stage), and post-germination (seedling) phases of soybean seed development using Illumina sequencing. In addition, we characterized the methylome of the cotyledons of germinated seedling to examine methylation differences before and after germination. Illumina sequencing of bisulfite-converted genomic DNA from globular stage (GLOB), cotyledon-stage (COT), early-maturation stage (EM), mid-maturation stage (MM), and late-maturation stage (LM), pre-dormancy stage 1 (PD1), pre-dormancy stage 2 (PD2), dormancy stage (DRY) seeds, and post-germination seedling (SDLG), seedling cotyledons (SDLG-COT) were studied.






Funded by the USDA-ARS. Developed by the USDA-ARS SoyBase and Legume Clade Database group at the Iowa State University, Ames, IA
 
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