SoyBase Follow us on Twitter @SoyBaseDatabase
Integrating Genetics and Genomics to Advance Soybean Research



Reference Report for SoyBase1802943
Title:A small gene family of broad bean codes for late nodulins containing conserved cysteine clusters
Authors:Fruehling, M., Albus, U., Hohnjec, N., Geise, G., Puehler, A., Perlick, A.M.
Source:Plant Sci. 2000, 152(1):67-77
Abstract:Five transcripts encoding different members of a nodulin family with conserved cysteine clusters (Cys-X4-Asp-Cys and Cys-X4-Cys) were identified in broad bean (Vicia faba cv. Kleine Thüringer) root nodules. They displayed homologies to the early nodulins PsENOD3 and PsENOD14 and the late nodulin PsNOD6 from pea. In addition to the occurrence of putative secretory signal peptides, the spatial distribution of the cysteine residues was comparable in both the broad bean and the pea nodulins. Based on tissue print hybridizations, it was found that the corresponding broad bean genes VfNOD-CCP1, VfNOD-CCP3 and VfNOD-CCP5 were expressed in the interzone II-III and the nitrogen fixing zone III of mature nodules, whereas the gene VfNOD-CCP4 was first induced in the prefixing zone II. A strong expression of the VfNOD-CCP2 gene only could be detected in the interzone II-III region. Sequence analysis of a genomic VfNOD-CCP1 clone revealed the presence of one intron separating a first exon encoding the signal peptide from a second exon encoding the cysteine cluster domain of this nodulin. Apart from the multiple presence of the common nodulin motifs AAAGAT and CTCTT on both DNA strands of the putative VfNOD-CCP1 promoter region, a sequence element resembling the organ specific element of the soyabean lbc3 gene promoter was identified. Nucleotide sequence data have been submitted to the EMBL/DDBJ/GenBank databases under the accession number AJ243461 and AJ243462 (VfNOD-CCP1), AJ243463 (VfNOD-CCP2), AJ243464 (VfNOD-CCP3), AJ243465 (VfNOD-CCP4) and AJ243466 (VfNOD-CCP5).






Funded by the USDA-ARS. Developed by the USDA-ARS SoyBase and Legume Clade Database group at the Iowa State University, Ames, IA
 
USDA Logo
Iowa State University Logo