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Integrating Genetics and Genomics to Advance Soybean Research

Experiment GSE12300
Title: Suppression of oleosin in soybean cotyledon
Description: 3 samples, 2 reps each. comparison of wildtype cotyledon to RNAi oleosin transgenic. Using RNAi, the seed oil body protein 24-kDa oleosin has been suppressed in transgenic soybeans. The endoplasmic reticulum (ER) forms micro-oil bodies about 50 nm in diameter that coalesce with adjacent oil bodies forming a hierarchy of oil body sizes. The oil bodies in the oleosin knockdown form large oil body-ER complexes with the interior dominated by micro-oil bodies and intermediate-sized oil bodies, while the peripheral areas of the complex are dominated by large oil bodies. The complex merges to form giant oil bodies with onset of seed dormancy that disrupts cell structure. The transcriptome of the oleosin knockdown shows few changes compared to wild-type. Proteomic analysis of the isolated oil bodies of the 24-kDa oleosin knockdown shows the absence of the 24-kDa oleosin and the presence of abundant caleosin and lipoxygenase. The formation of the micro-oil bodies in the oleosin knockdown is interpreted to indicate a function of the oleosin as a surfactant.
Platform: GPL4592
Type: Expression_GeneChip
Class: comparative
Publication: Suppression of soybean oleosin produces micro-oil bodies that aggregate into oil body/ER complexes.(Schmidt et al. 2008a)
GEO:View Experiment Records At GEO

Funded by the USDA-ARS. Developed by the USDA-ARS SoyBase and Legume Clade Database group at the Iowa State University, Ames, IA
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