Soybean Fast Neutron Mutants

Description:

Compiled here are over 20,000 independent soybean fast neutron mutant lines derived from M92-220 developed for use in soybean functional genomics. Within the catalog are mutants with observed phenotypic variation in seed composition, maturity, morphology, pigmentation, roots and nodulation. This population was first used for a forward screen of seed protein and oil mutants. In the meantime, it was revitalized as a resource for phenome analysis and future forward and reverse genetics screens. Analysis of genomic DNA from a subset of mutants for deletions and additions by comparative genome hybridization are also displayed on this site.

Publications:

Citation: Bolon YT, Haun WJ, Xu WW, Grant D, Stacey MG, Nelson RT, Gerhardt DJ, Jeddeloh JA, Stacey G, Muehlbauer GJ, Orf JH, Naeve SL, Stupar RM, Vance CP. Phenotypic and genomic analyses of a fast neutron mutant population resource in soybean. Plant Physiol. 2011 May;156(1):240-53. doi: 10.1104/pp.110.170811. Epub 2011 Feb 14. PMID: 21321255; PMCID: PMC3091049.

Publication link: 10.1104/pp.110.170811

Citation: Bolon YT, Stec AO, Michno JM, Roessler J, Bhaskar PB, Ries L, Dobbels AA, Campbell BW, Young NP, Anderson JE, Grant DM, Orf JH, Naeve SL, Muehlbauer GJ, Vance CP, Stupar RM. Genome resilience and prevalence of segmental duplications following fast neutron irradiation of soybean. Genetics. 2014 Nov;198(3):967-81. doi: 10.1534/genetics.114.170340. Epub 2014 Sep 10. PMID: 25213171; PMCID: PMC4224183.

Publication link: 10.1534/genetics.114.170340

Data Links:

Also see examples of the contents of each file here.

• Fasta file of FN deletions from the Wm82.a1.v1 / Wm82.gnm1.ann1 assembly
• insertion_event.tsv -- Insertion IDs, names, vectors
• insertion_mutant.tsv -- Insertion line and responsible lab
• insertion_mutant_props.tsv -- Phenotypic effects in mutant lines
• insertion_mutant_synonym.tsv -- Mutant line name synonyms
• insertion_position.tsv -- Insertion positions, Wm82.a2.v1
• mutant_cgh_experiment_genes_table.tsv -- Genes affected by mutations (Wm82.a1.v1)
• mutant_cgh_experiment_indel_table.tsv -- Mutant indel locations (Wm82.a1)
• mutant_cgh_experiment_indel_table_v2.tsv -- Mutant indel locations; new identifiers
• mutant_cgh_experiment_locus_table.tsv -- SSR markers in indel regions
• mutant_descriptor_xref_table.tsv -- SoyBase trait names and IDs
• mutant_descriptors_table.tsv -- Mutant phenotype descriptions
• mutant_experiment_table.tsv -- Mutation experiments
• mutant_files_table.tsv -- Mutants and image names
• mutant_name_translation_table.tsv -- Mutant name correspondences (Soybase ID, sample ID, truncated sample ID)
• mutant_samples_table.tsv -- Experiment sample IDs, seed availability, cultivar names
• mutant_trait_values_table.tsv -- Mutation IDs and traits

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