Genome-wide characterization and expression analysis of TOPP-type protein phosphatases in soybean (Glycine max L.) reveal the role of GmTOPP13 in drought tolerance.

Reference Report for AP20210611.2

Title:	Genome-wide characterization and expression analysis of TOPP-type protein phosphatases in soybean (Glycine max L.) reveal the role of GmTOPP13 in drought tolerance.
Authors:	Wang, S., Guo, J., Zhang, Y., Guo, Y., Ji, W.
Source:	Wang et al.2021b Genes Genom
Abstract:	Background In response to various abiotic stressors such as drought, many plants engage different protein phosphatases linked to several physiological and developmental processes. However, comprehensive analysis of this gene family is lacking for soybean. Objective This study was performed to identify the TOPP-type protein phosphatase family in soybean and investigate the genes role under drought stress. Methods Soybean genome sequences and transcriptome data were downloaded from the Phytozome v.12, and the microarray data were downloaded from NCBI GEO datasets GSE49537. Expression profiles ofGmTOPP13were obtained based on qRT-PCR results.GmTOPP13gene was transformed into tobacco plants viaAgrobacteriummediated method, and the drought tolerance was analyzed by water deficit assay. Results 15GmTOPPgenes were identified in the soybean genome database (GmTOPP115).GmTOPPgenes were distributed on 9 of 20 chromosomes, with similar exon-intron structure and motifs arrangement. All GmTOPPs contained Metallophos and STPPase_N domains as well as the core catalytic sites. Cis-regulatory element analysis predicted thatGmTOPPswere widely involved in plant development, stress and hormone response in soybean. Expression profiles showed thatGmTOPPsexpressed in different tissues and exhibited divergent expression patterns in leaf and root in response to drought stimulus. Moreover,GmTOPP13gene was isolated and expression pattern analysis indicated that this gene was highly expressed in seed, root, leaf and other tissues detected, and intensively induced upon PEG6000 treatment. In addition, overexpression ofGmTOPP13gene enhanced the drought tolerance in tobacco plants. The transgenic tobacco plants showed regulation of stress-responsive genes including CAT, SOD, ERD10B and TIP during drought stress. Conclusions This study provides valuable information for the study ofGmTOPPgene family in soybean, and lays a foundation for further functional studies ofGmTOPP13gene under drought and other abiotic stresses.

Funded by the USDA-ARS. Developed by the USDA-ARS SoyBase and Legume Clade Database group at the Iowa State University, Ames, IA

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