Reference Report for AP20210622.3
| Title: | A VIT-like transporter facilitates iron transport into nodule symbiosomes for nitrogen fixation in soybean. |
| Authors: | Liu, S., Liao, L.L., Nie, M.M., Peng, W.T., Zhang, M.S., Lei, J.N., Zhong, Y.J., Liao, H., Chen, Z.C. |
| Source: | Liu et al. 2020 New Phytol, 226:1413-1428 |
| Abstract: | Effective legumerhizobia symbiosis depends on efficient nutrient exchange. Rhizobia need to synthesize iron-containing proteins for symbiotic nitrogen fixation (SNF) in nodules, which depends on host plant-mediated iron uptake into the symbiosome. We functionally investigated a pair of vacuolar iron transporter like (VTL) genes,GmVTL1a/b, in soybean (Glycine max) and evaluated their contributions to SNF, including investigations of gene expression patterns, subcellular localization, and mutant phenotypes.Though bothGmVTL1a/bgenes were specifically expressed in the fixation zone of the nodule, GmVTL1a was the lone member to be localized at the tonoplast of tobacco protoplasts, and shown to facilitate ferrous iron transport in yeast. GmVTL1a targets the symbiosome in infected cells, as verified byin situimmunostaining. Twovtl1knockout mutants had lower iron concentrations in nodule cell sap and peribacteroid units than in wild-type plants, suggesting thatGmVTL1knockout inhibited iron import into symbiosomes. Furthermore,GmVTL1knockout minimally affected soybean growth under nonsymbiotic conditions, but dramatically impaired nodule development and SNF activity under nitrogen-limited and rhizobia-inoculation conditions, which eventually led to growth retardation.Taken together, these results demonstrate that GmVTL1a is indispensable for SNF in nodules as a transporter of ferrous iron from the infected root cell cytosol to the symbiosome. |
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