Reference Report for AP20210622.4
| Title: | GmWRKY45 enhances tolerance to phosphate starvation and salt stress, and changes fertility in transgenic Arabidopsis. |
| Authors: | Li, C., Liu, X., Ruan, H., Zhang, J., Xie, F., Gai, J., Yang, S. |
| Source: | Li et al. 2020 Front. Plant Sci., 10:1714 |
| Abstract: | WRKY protein is a unique transcription factor (TF) and plays an important role in the physiological processes of various stress responses and plant development. In this research, we obtained a WRKY TF gene from soybean by homologous cloning, and named itGmWRKY45.GmWRKY45is a nuclear protein containing a highly conserved WRKY domain and a C2H2zinc finger structure, and mainly expressed in roots, flowers and pods of soybean. The quantitative reverse transcriptionPCR showed thatGmWRKY45was induced by phosphate starvation and salt stress. As compared with the wild type (WT), overexpression ofGmWRKY45increased the adaptability of transgenicArabidopsisto phosphate starvation, which might be related to the enhancement of lateral root development. The phosphorus concentration, fresh weight and dry weight ofGmWRKY45-overexpressingArabidopsiswere higher than those of WT under Pi-sufficient or Pi-deficient condition. Meantime, the expression of phosphate-responsive genes was affected in transgenicArabidopsis.Furthermore,GmWRKY45improved the salt tolerance and changed fertility of transgenicArabidopsis. Under salt stress, we found the survival rate and soluble sugar content of transgenicArabidopsiswere significantly higher than those of WT. In a conventional soil pot experiment, the transgenicArabidopsisproduced shorter silique, less and larger seeds than WT, these might be due to partial abortion of pollens. The overall results showed thatGmWRKY45was not only involved in response to abiotic stress but also related to fertility, suggested thatGmWRKY45had an elaborate regulatory system in plants. |
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