A DnaJ protein that interacts with soybean mosaid virus coat protein serves as a key susceptibility factor for viral infection

Reference Report for AP20210629.4

Title:	A DnaJ protein that interacts with soybean mosaid virus coat protein serves as a key susceptibility factor for viral infection
Authors:	Zong, T., Yin, J., Jin, T., Wang, L., Luo, M., Li, K., Zhi, H.
Source:	Zong et al. 2020 Virus Reasearch, 281:197870
Abstract:	Soybean mosaic virus (SMV)-disease is one of the most serious and widespread diseases in soybean (Glycine max). In the present study, a DnaJ protein in soybean designated GmCPIP (SMV coat protein-interacting protein) was screened by the QIS-Seq (quantitative interactor screening with next-generation sequencing) method, and the interaction between SMV CP and GmCPIP was confirmed by the yeast two-hybrid (Y2H) system and bimolecular fluorescence complementation (BiFC) assay. Subcellular localization analysis indicated that both proteins are localized in the cytoplasm, cytomembrane and nucleus. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that infection with SMV-SC4 temporarily increased the transcription of GmCPIP. Virus-induced gene silencing (VIGS) down-regulated the GmCPIP gene by 82%, and the accumulation of SMV was decreased by 88.6% in GmCPIP-silenced plants inoculated with SMV-SC4. The interaction of GmCPIP with SMV CP seems to contribute to SMV infection in soybean.

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