Reference Report for AP20210701.2
| Title: | GmWRKY40, a member of the WRKY transcription factor genes identified from Glycine max L., enhanced the resistance to Phytophthora sojae |
| Authors: | Cui, X., Yan, Q., Gan, S., Xue, D., Wang, H., Xing, H., Zhao, J., Guo, N, |
| Source: | Cui et al. 2019 BMC Plant Biol. |
| Abstract: | Background The WRKY proteins are a superfamily of transcription factors and members play essential roles in the modulation of diverse physiological processes, such as growth, development, senescence and response to biotic and abiotic stresses. However, the biological roles of the majority of the WRKY family members remains poorly understood in soybean relative to the research progress in model plants. Results In this study, we identified and characterizedGmWRKY40, which is a group IIcWRKYgene. Transient expression analysis revealed that the GmWRKY40 protein is located in the nucleus of plant cells. Expression ofGmWRKY40was strongly induced in soybean following infection withPhytophthora sojae, or treatment with methyl jasmonate, ethylene, salicylic acid, and abscisic acid. Furthermore, soybean hairy roots silencingGmWRKY40enhanced susceptibility toP. sojaeinfection compared with empty vector transgenic roots. Moreover, suppression ofGmWRKY40decreased the accumulation of reactive oxygen species (ROS) and modified the expression of several oxidation-related genes. Yeast two-hybrid experiment combined with RNA-seq analysis showed that GmWRKY40 interacted with 8 JAZ proteins with or without the WRKY domain or zinc-finger domain of GmWRKY40, suggesting there were different interaction patterns among these interacted proteins. Conclusions Collectively, these results suggests thatGmWRKY40functions as a positive regulator in soybean plants response toP. sojaethrough modulating hydrogen peroxide accumulation and JA signaling pathway. |
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