Reference Report for AP20210713.6
| Title: | Genome-Wide analysis of the DYW subgroup PPR gene family and identification of GmPPR4 responses to drought stress |
| Authors: | Su, H., Li, B., Song, X., Ma, J., Chen, J., Zhou, Y., Chen, M., Min, D., Xu, Z., Ma, Y. |
| Source: | Su et al. 2019 Int. J. Mol. Sci., 20(22): 5667 |
| Abstract: | Pentatricopeptide-repeat (PPR) proteins were identified as a type of nucleus coding protein that is composed of multiple tandem repeats. It has been reported thatPPRgenes play an important role in RNA editing, plant growth and development, and abiotic stresses in plants. However, the functions of PPR proteins remain largely unknown in soybean. In this study, 179 DYW subgroupPPRgenes were identified in soybean genome (Glycine maxWm82.a2.v1). Chromosomal location analysis indicated that DYW subgroupPPRgenes were mapped to all 20 chromosomes. Phylogenetic relationship analysis revealed that DYW subgroupPPRgenes were categorized into three distinct Clusters (I to III). Gene structure analysis showed that mostPPRgenes were featured by a lack of intron. Gene duplication analysis demonstrated 30PPRgenes (15 pairs; ~35.7%) were segmentally duplicated among Cluster IPPRgenes. Furthermore, we validated the mRNA expression of three genes that were highly up-regulated in soybean drought- and salt-induced transcriptome database and found that the expression levels ofGmPPR4were induced under salt and drought stresses. Under drought stress condition,GmPPR4-overexpressing (GmPPR4-OE) plants showed delayed leaf rolling; higher content of proline (Pro); and lower contents of H2O2, O2and malondialdehyde (MDA) compared with the empty vector (EV)-control plants.GmPPR4-OE plants exhibited increased transcripts of several drought-inducible genes compared with EV-control plants. Our results provided a comprehensive analysis of the DYW subgroupPPRgenes and an insight for improving the drought tolerance in soybean. |
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