Reference Report for AP20210730.2
| Title: | Overexpression of GmCAMTA12 enhanced drought tolerance in Arabidopsis and soybean. |
| Authors: | Noman, M., Jameel, A., Qiang, W., Ahmad, N., Liu, W., Wang, F., Li, H. |
| Source: | Noman et al. 2109 Int. J. Mol. Sci., 20(19): 4849 |
| Abstract: | Fifteen transcription factors in the CAMTA (calmodulin binding transcription activator) family of soybean were reported to differentially regulate in multiple stresses; however, their functional analyses had not yet been attempted. To characterize their role in stresses, we first comprehensively analyzed theGmCAMTAfamily in silico and thereafter determined their expression pattern under drought. The bioinformatics analysis revealed multiple stress-relatedcis-regulatory elements includingABRE,SARE,G-boxandW-box, 10 unique miRNA (microRNA) targets inGmCAMTAtranscripts and 48 proteins in GmCAMTAs interaction network. We then cloned the 2769 bp CDS (coding sequence) ofGmCAMTA12in an expression vector and overexpressed in soybean and Arabidopsis throughAgrobacterium-mediated transformation. The T3 (Transgenic generation 3) stably transformed homozygous lines of Arabidopsis exhibited enhanced tolerance to drought in soil as well as on MS (Murashige and Skoog) media containing mannitol. In their drought assay, the average survival rate of transgenic Arabidopsis lines OE5 and OE12 (Overexpression Line 5 and Line 12) was 83.66% and 87.87%, respectively, which was ~30% higher than that of wild type. In addition, the germination and root length assays as well as physiological indexes such as proline and malondialdehyde contents, catalase activity and leakage of electrolytes affirmed the better performance of OE lines. Similarly,GmCAMTA12overexpression in soybean promoted drought-efficient hairy roots in OE chimeric plants as compare to that of VC (Vector control). In parallel, the improved growth performance of OE in Hoagland-PEG (polyethylene glycol) and on MS-mannitol was revealed by their phenotypic, physiological and molecular measures. Furthermore, with the overexpression ofGmCAMTA12, the downstream genes includingAtAnnexin5,AtCaMHSP,At2G433110andAtWRKY14were upregulated in Arabidopsis. Likewise, in soybean hairy roots,GmELO,GmNABandGmPLA1-IIdwere significantly upregulated as a result ofGmCAMTA12overexpression and majority of these upregulated genes in both plants possess CAMTA bindingCGCG/CGTGmotif in their promoters. Taken together, we report thatGmCAMTA12plays substantial role in tolerance of soybean against drought stress and could prove to be a novel candidate for engineering soybean and other plants against drought stress. Some research gaps were also identified for future studies to extend our comprehension ofCa-CaM-CAMTA-mediated stress regulatory mechanisms. |
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