Genetic independence of the nodulation-response gene loci--Rj1, Rj2, and Rj4--in soybean.

Reference Report for IND20405186

Title:	Genetic independence of the nodulation-response gene loci--Rj1, Rj2, and Rj4--in soybean.
Authors:	Devine, T.E., O'Neill, J.J.
Source:	J. Hered. 1993, 84(2):140-142
Abstract:	Several of the soybean genes controlling leghemoglobin synthesis during nodule development have been reported to occur in two gene clusters. Several of the genes controlling avirulence for rust resistance in flax are genetically linked. This study was undertaken to determine if there was any evidence of linkage or clustering among the Rj1, Rj2, and Rj4 genes controlling nodulation in soybean. Hybridizations were made in the field at Beltsville, Maryland, and F1 plants were grown in the greenhouse to produce F2 seeds. F2 plants were grown in the field and individually harvested to obtain F3 seeds, The F3 seeds were planted in the field or in growth trays to assay for the Rj1 versus rj1 alleles. To assay for the Rj2 versus rj2 and Rj4 versus rj4 alleles, the F3 seeds were planted in vermiculite in growth trays in the greenhouse and inoculated with bradyrhizobial strain USDA 7 or USDA 61, respectively. Linkage chi-square values for the comparisons Rj1 versus Rj2, Ri1 versus Rj4, and Rj2 versus Rj4 indicated no evidence of genetic linkage or clustering of these genes.

Funded by the USDA-ARS. Developed by the USDA-ARS SoyBase and Legume Clade Database group at the Iowa State University, Ames, IA

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