Reference Report for IND20512741
| Title: | Differential induction and accumulation of beta-1,3-glucanase and chitinase isoforms in soybean hypocotyls and leaves after compatible and incompatible infection with Phytophthora megasperma f.sp. glycinea. |
| Authors: | Yi, S.Y., Hwang, B.K. |
| Source: | Physiol. Mol. Plant Pathol. 1996, 48(3):179-192 |
| Abstract: | Phytophthora megasperma f.sp. glycinea infection greatly induced the synthesis and accumulation of beta 1,3-glucanases and chitinases in hypocotyls and leaves of soybean seedlings. Native polyacrylamide gel electrophoresis and isoelectric focusing were used to identify and quantify the isoforms of the two hydrolases in soybean tissues. Some beta-1,3-glucanase isoforms were constitutively expressed in soybeans, but other isoforms only accumulated in soybean or only in a particular organ of soybean after inoculation with P. m. f.sp. glycinea infection or after treatment with mercuric chloride. The pI 8.4 isoform, Gb 2 of beta-1,3-glucanase constitutively present in soybean hypocotyls and leaves completely disappeared after mercuric chloride treatment. P. m. f.sp. glycinea infection and mercuric chloride treatment leads to the accumulation of distinctly different isoforms of chitinase in soybean tissue. The acidic chitinase isoform Ca 4 was induced and accumulated in hypocotyls, but not in leaves, after pathogen infection and mercuric chloride treatment. In general, beta-1,3-glucanase and chitinase isoforms accumulated in the diseased soybean tissues during symptom development in both compatible and incompatible interactions. The results also suggest that pathogen infection and chemical stress may function in a different organ-specific manner to induce isoforms of beta-1,3-glucanase and chitinase in soybeans. |
SoyBase
Follow us on Twitter @SoyBaseDatabase
