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Reference Report for IND20527700
Title:The use of RAPD amplified DNA as markers for virulence characteristics in soybean cyst nematode.
Authors:Li, J., Faghihi, J., Ferris, J.M., Ferris, V.R.
Source:Fund. Appl. Nematol. 1996, 19(2):143-150
Abstract:The feasibility was investigated of developing RAPD-based diagnostic dot blot tests to separate field isolates of soyabean cyst nematode (Heterodera glycines) with different virulence to resistant cultivars of soyabean. A RAPD primer was found that separated two northern Indiana isolates of the nematode with similar virulence toward resistant soyabean lines, from two southern Indiana isolates with different virulence characteristics; and a molecular marker probe was developed for use with dot blots. The study demonstrates the feasibility of developing a rapid-based test for use in diagnosing larger groups of field isolates of nematodes that share common virulence characteristics toward resistant cultivars.






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