Reference Report for IND23281015
| Title: | Flow cytometric analysis of Phialophora gregata isolated from soybean plants resistant and susceptible to brown stem rot |
| Authors: | Yeater, K.M., Grau, C.R., Rayburn, A.L. |
| Source: | Phytopath. 2002, 150 (4-5):258-262 |
| Abstract: | Flow cytometry was used to characterize isolates of Phialophora gregata, the causal organism of brown stem rot (BSR) in soyabean, using the fluorescence intensity of YOYO-1 stained conidia. The conidia were derived from isolates of P. gregata isolated from field-grown soyabean vascular tissue from two locations (Arlington and Hancock Agricultural Research Stations) in Wisconsin, USA. At each location, four soyabean cultivars were grown in 1996. Two cultivars (Sturdy and AP1991) were susceptible to BSR and two cultivars (Bell and BSR101) were resistant to BSR. Ninety-six isolates, one per plant, were characterized for DNA content. The stained conidia were analysed with an EPICS XL (Coulter Corporation, Hialeah, Florida, USA) flow cytometer cell sorter with CICERO software (San Rafael, California, USA). The isolates differed in their mean DNA content, ranging from 78.0 to 116.8 arbitrary units. Statistical analysis showed a significant difference in the DNA content variability of the isolates between the two locations. A notable difference in DNA content variability was observed between the isolates collected from the resistant cultivars between the two locations. However, no significant variation occurred in the DNA content of the isolates collected from susceptible cultivars within or between locations. The observed variation among the isolates is hypothesized to be due to the occurrence of separate populations of P. gregata and selection for mean DNA content that could colonize specific plant genotypes. |
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