Reference Report for IND86060304
Title: | Soybean yellow vein, a new virus disease of soybean. |
Authors: | Senboku, T., Kittipakorn, K., Srithongchai, W., Thongeerkom, P., Deema, N., Kiratiya-Angul, S. |
Source: | Trop. Agric. Res. Ser. 1986, No. 19:101-107 |
Abstract: | A virus isolated from soyabean plants showing yellow vein symptoms in central Thailand in 1982 was previously unknown on this host and was designated soyabean yellow vein virus. Distribution was limited to a field where soyabean was cultivated in rotation with sorghum. Numerous rod-shaped virus particles (c. 500-550 nm × 15-20 nm) were detected in dip preparations of infected soyabean leaves. Chenopodium amaranticolor and C. quinoa showed distinct chlorotic local lesions on inoculated leaves and only soyabean was recognized as a systemically infected host. Attempts to transmit SYVV by insects failed. The dilution end point was 10-3-10-4, thermal inactivation 35-40°C (10 min) and longevity in vitro 2-3 h at 4°. Attempts to obtain a purified preparation of SYVV were made. After sucrose density gradient centrifugation, the peak fraction identified in the relative absorbance curve with UV light (254 nm absorbance zone) showed the highest infectivity and also contained numerous rod-shaped particles. |