Identification of dsRNAs associated with soybean dwarf virus-infected soybean.

Reference Report for IND91014500

Title:	Identification of dsRNAs associated with soybean dwarf virus-infected soybean.
Authors:	Smith, O.P., Hunst, P.L., Hewings, A.D., Stone, A.L., Tolin, S.A., Damsteegt, V.D.
Source:	Phytopath. 1991, 81(2):131-134
Abstract:	Double-stranded (ds) RNA was isolated from leaves of soybean cultivar Wayne infected with the dwarfing (D) or yellowing (Y) strain of soybean dwarf virus (SDV). Each strain produced two virus-specific dsRNAs. SDV-D dsRNAs were estimated to have molecular weights of 3.4 and 1.9 X 10(6) Da. The corresponding SDV-Y species were larger, having estimated molecular weights of 3.6 and 2.2 X 10(6) Da. Northern blot hybridization analyses showed the strains are related and that the two dsRNAs correspond to viral genomic-length and 3' subgenomic-length species. The detection of SDV dsRNA by polyacrylamide gel electrophoresis (dsRNA profiles) was improved by combining S1 nuclease with DNase in a one-step treatment of nucleic acids isolated by CF-11 column chromatography to remove contaminating single-stranded RNA. The size-specificity of SDV-D and SDV-Y dsrnas was shown to be a useful phenotypic marker for in vitro strain differentiation.

Funded by the USDA-ARS. Developed by the USDA-ARS SoyBase and Legume Clade Database group at the Iowa State University, Ames, IA

U.S. Public Domain