Reference Report for IND93028975
| Title: | Effects of light- and altered-cercosporin phenotypes on gene expression in Cercospora kikuchii. |
| Authors: | Rollins, J.A., Ehrenshaft, M., Upchurch, R.G. |
| Source: | Can. J. Microbol. 1993, 39(1):118-124 |
| Abstract: | Cercospora kikuchii is a fungal pathogen of soybean that produces a photosensitizing and phytotoxic polyketide, cercosporin, in culture and in planta. We have studied the influence of growth stage, light, and growth stage, light, and growth medium on cercosporin accumulation in wild-type isolate and three mutant strains with altered toxin phenotypes. After an initial logarithmic growth phase, the wild-type isolate accumulated high levels of cercosporin on either complete medium or potato dextrose broth, but only when cultured in the light. Dark-grown cultures of the wild-type and light-grown cultures of two uv-induced mutant derivatives accumulated 100-fold lower cercosporin levels. A third mutant stain accumulated wild-type cercosporin levels, but only when cultured in the light in potato dextrose broth. Two-dimensional gel electrophoresis of both extracted proteins and in vitro translation products from wild-type cultures revealed the presence of polypeptides and poly A + RNAs whose accumulation was positively regulated by light. Comparison of translated polypeptide patterns from wild-type and mutant cultures also demonstrated differential accumulation of translatable poly A + RNAs in cercosporin-producing and nonproducing cultures |
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