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Reference Report for KGK20170519.1
Title:Identification of Quantitative Trait Loci Underlying Seed Protein Content of Soybean including Main, Epistatic and QTL _ Environment Effects in Different Regions of Northeast China
Authors:Teng, W., Li, W., Zhang, Q., Wu, D., Zhao, X., Li, H., Han, Y., Li, W.
Source:Genome. 2017
Abstract:The objective here was to identify QTL, their epistatic effects and QTLs x environments interaction underlying soybean protein content (PC). The mapping population, consisted of 129 recombinant inbred lines (RILs), was created by crossing ÔDongnong46Õ and ÔL-100Õ. Phenotypic data of the parents and RILs were collected for four years in three locations of Heilongjiang Province of China. A total of 213 simple sequence repeat markers were used to construct a genetic linkage map. Eight QTLs, located on seven chromosomes (Chr), were identified to be associated with PC among the ten tested environments. Of the seven QTLs, five QTLs, qPR-2 (Satt710, on Chr9), qPR-3 (Sat_122, on Chr12), qPR-5 (Satt543, on Chr17), qPR-7 (Satt163, on Chr18) and qPR-8 (Satt614, on Chr20), were detected in 6, 7, 7, 6 and 7 environments, respectively, implying relatively stable QTLs. qPR-3 could explain 3.33-11.26% of the phenotypic variation across eight tested environments. qPR-5 and qPR-8 explained 3.64 to10.1% and 11.86 to18.40% of the phenotypic variation, respectively, across seven tested environments. Eight QTLs associated with PC exhibited additive and/or additive _ environment interaction effects. The results showed that environment-independent QTLs often had higher additive effects. Moreover, five epistatic pairwise QTLs were identified in the ten environments.






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