Reference Report for SoyBase102798449
| Title: | Peanut stunt virus isolated from soybeans, Glycine max Merr |
| Authors: | Iizuka, N., Yunoki, T. |
| Source: | Bull. Toho. Natl. Ag. Exp. Stn. 1974, 47:1-12 |
| Abstract: | Groundnut stunt virus was isolated from a soybean plant showing stunt and mosaic. The main symptoms in soybean are mottling and crinkling of the leaves, and stunting. The mottle pattern on seed differed from that caused by soybean mosaic or stunt viruses. The virus infected 20 of 41 soybean vars. tested and had a wide host range. It was transmitted by the bean aphid (Aphis craccivora) in glasshouse tests and in 3-4% of the seeds from 2 of 3 vars. tested. It was not transmitted through bean (?Phaseolus vulgaris) or cowpea seeds. In infected cowpea juice it was inactivated after 10 min at 60-65 deg C; longevity in vitro was 1-2 days at 20 deg and dilution end point was near 1:10 000. Negative results of cross protection were obtained between the virus and cucumber mosaic, soybean stunt or chrysanthemum mild mottle viruses. Serological tests with the purified virus showed it to be closely related to GSV W and GSV J. Purified virus reacted partially with antisera to CMV Y and SSV but not with CMMV. Electron microscopy revealed spherical particles 25-28 nm diam. and aggregation of particles in soybean leaves in the cytoplasm and vacuole. |
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