Reference Report for SoyBase102898944
| Title: | DIFFERENTIATION OF BIOLOGICALLY DISTINCT PEANUT STRIPE POTYVIRUS STRAINS BY A NUCLEOTIDE POLYMORPHISM-BASED ASSAY |
| Authors: | Pappu, S.S., Pappu, H.R., Chang, C.A., Culbreath, A.K., Todd, J.W. |
| Source: | Plant Dis. 1998, 82(10):1121-1125 |
| Abstract: | A necrotic strain of peanut stripe potyvirus (PStV-Ts) was used to design and test strain-differentiating oligonucleotides. The 3′ region of PStV-Ts, including a part of the NIb region, the complete coat protein (CP) gene, and the 3′-untranslated region, were cloned and sequenced. PStV-Ts had a high degree of sequence identity (92-95%) to the known non-necrotic (blotch) strains, both at the nucleotide and amino acid sequence levels. Nucleotide sequence differences unique to the necrotic strain were identified when compared to the available non-necrotic isolates of PStV. Nucleotide polymorphism in the CP gene sequences was utilized in designing oligonucleotides that were specific to the necrotic strain, and were employed in an assay to differentiate the necrotic strain from non-necrotic. The 3′ end mismatch in the oligonucleotides contributed in particular to the differentiation of the strains. It is concluded that this approach facilitated rapid, sensitive and reliable detection and differentiation of PStV strains. |
SoyBase
Follow us on Twitter @SoyBaseDatabase
