Reference Report for SoyBase112981045
| Title: | Detection of cross-reactive antigens shared by Fusarium oxysporum and Glycine max by indirect elisa and their cellular location in root tissues |
| Authors: | Chakraborty, B.N., Sarkar, B., Chakraborty, U. |
| Source: | Folia Microbiol. 1997, 42(6):607-612 |
| Abstract: | Pathogenicity tests were conducted in the Phytopathological Experimental Garden, Department of Botany, University of North Bengal, India, to screen 10 cultivars of soyabean against F. oxysporum. The results revealed Soymax and Punjab-1 to be most resistant while JS-2 and UPSM-19 were most susceptible. Antigens were prepared from the roots of all 10 varieties of soyabean and the mycelium of F. oxysporum. Polyclonal antisera were raised against a mycelial suspension of F. oxysporum and the root antigen of the susceptible cultivar UPSM-19. Cross reactive antigens (CRAs) shared by the host and the pathogen were detected first by immunodiffusion. The immunoglobulin fraction of the antiserum was purified by ammonium sulfate precipitation and DEAE-Sephadex column chromatography. The immunoglobulin fractions were used for detection of CRAs by ELISA. In ELISA, antigens of susceptible cultivars showed higher absorbance values when tested against the purified anti-F. oxysporum antiserum. Antiserum produced against UPSM-19 showed cross-reactivity with the antigens of other cultivars. Indirect staining of antibodies using fluorescein isothiocyanate indicated that in cross-sections of roots of a susceptible cultivar (UPSM-19) CRAs were concentrated around xylem elements, endodermis and epidermal cells, while in the resistant variety, fluorescence was concentrated mainly around epidermal cells and distributed in the cortical tissues. CRAs were also present in microconidia, macroconidia and chlamydospores of the fungus. |
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