Reference Report for SoyBase121991123
| Title: | Mapping of duplicate genes in soybean |
| Authors: | Lee, J.M., Bush, A.L., Specht, J.E., Shoemaker, R.C. |
| Source: | Genome 1999, 42(5):829-836 |
| Abstract: | Appressed pubescence genes in soyabean cause hairs on the upper surface of leaves to lie flat, while pubescence remains erect elsewhere on the plant. For decades this trait was believed to be controlled in soyabean by duplicated single genes, Pa1 and Pa2. However, reports in the literature conflicted as to which phenotype was dominant or recessive. Two populations were developed, each of approximately 100 individuals, and each segregating for one of the appressed pubescence genes. A combination of SSRs (simple sequence repeats) and RFLPs (restriction fragment length polymorphisms) was used in each of these populations to map the independent genes. Two-point analysis weakly linked Pa1 and Pa2 to separate linkage groups. Lack of strong linkage suggested the trait may not be controlled by single genes. When QTL (quantitative trait loci) analysis was performed, one major locus and several minor loci were detected in each population. This paper reports the mapping of the genes controlling appressed pubescence in soyabean and their placement in homologous regions. Although appressed pubescence was originally reported to be single duplicate genes, the results obtained here indicated that it is actually a more complex phenotype with major duplicated genes and minor modifying genes. These results offer interesting implications regarding the evolution of duplicate genetic factors and the definition of qualitative traits. |
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