Reference Report for SoyBase73001959
| Title: | Biological and physicochemical properties of cowpea severe mosaic comovirus isolated from soybean in the State of Parana |
| Authors: | Bertacini, P.V., Almeida, A.M.R., Lima, J.A.A., Chagas, C.M. |
| Source: | Braz. Arch. Biol. Technol. 1998, 41(4):409-416 |
| Abstract: | Soyabean plants in Paraná, Brazil, with symptoms of bud blight were observed growing close to cowpea plants with severe symptoms of mosaic associated with blisters in the leaves. A group of plants of both species were collected and used for aetiological studies. Bud blight symptoms induced by tobacco streak ilarvirus were common in soyabeans in certain areas of Paraná. Host range, serological reaction, particle morphology and size, protein and nucleic acid analysis and transmission by beetles (Cerotoma arcuata) showed that the virus involved was cowpea severe mosaic comovirus. This is the first report of the occurrence of this virus in soyabean plants in Paraná. Results using indirect ELISA showed that in cowpea the relative virus concentration was higher in green leaf areas than in chlorotic leaf areas. Virus concentration, determined through indirect ELISA, was much higher in plants kept in a diurnal regime of 25°C×23° (12×12 h) than at 30°×28°. |
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