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Reference Report for SoyBase8101930
Title:Purification of blackgram mottle virus (BMoV) using magnesium-bentonite
Authors:Balasubrahmanyam, A., Kapoor, H.C., Varma, A.
Source:Phytochem. 1997, 44(7):1237-1240
Abstract:Blackgram mottle virus was purified from French bean (Phaseolus vulgaris) cv. Contender tissue using two different methods involving differential centrifugation. The use of Mg-bentonite during purification produced a homogeneous and pure virus preparation. The Mr of RNA and coat protein were 1400 and 39k, respectively. The RNA was infectious at a concentration as low as 7 micrograms ml(-1).






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